
                                SEQUENCE LISTING

<110> Wagner, Thor
      Scharenberg, Andrew M.
      Rawlings, David J.

<120> CCR5 DISRUPTION OF CELLS EXPRESSING
  ANTI-HIV CHIMERIC ANTIGEN RECEPTOR (CAR) DERIVED FROM
  BROADLY NEUTRALIZING ANTIBODIES
  

<130> SCRI.076WO

<150> 62/059,723         
<151> 2014-03-10

<150> 61/985,947        
<151> 2014-04-29

<160> 15

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 257
<212> PRT
<213> Artificial Sequence

<220> 
<223> PGT128-scFV

<400> 1
Gln Ser Ala Leu Thr Gln Pro Pro Ser Ala Ser Gly Ser Pro Gly Gln
 1               5                  10                  15      
Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Asn Asn Phe Val Ser Trp
            20                  25                  30          
Tyr Gln Gln His Ala Gly Lys Ala Pro Lys Leu Val Ile Tyr Asp Val
        35                  40                  45              
Asn Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser
    50                  55                  60                  
Gly Asn Thr Ala Ser Leu Thr Val Ser Gly Leu Gln Thr Asp Asp Glu
65                  70                  75                  80  
Ala Val Tyr Tyr Cys Gly Ser Leu Val Gly Asn Trp Asp Val Ile Phe
                85                  90                  95      
Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Ser Gly Gly Gly Gly Ser
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Pro Gln Leu Gln Glu
        115                 120                 125             
Ser Gly Pro Thr Leu Val Glu Ala Ser Glu Thr Leu Ser Leu Thr Cys
    130                 135                 140                 
Ala Val Ser Gly Asp Ser Thr Ala Ala Cys Asn Ser Phe Trp Gly Trp
145                 150                 155                 160 
Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Val Gly Ser Leu Ser
                165                 170                 175     
His Cys Ala Ser Tyr Trp Asn Arg Gly Trp Thr Tyr His Asn Pro Ser
            180                 185                 190         
Leu Lys Ser Arg Leu Thr Leu Ala Leu Asp Thr Pro Lys Asn Leu Val
        195                 200                 205             
Phe Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr
    210                 215                 220                 
Cys Ala Arg Phe Gly Gly Glu Val Leu Arg Tyr Thr Asp Trp Pro Lys
225                 230                 235                 240 
Pro Ala Trp Val Asp Leu Trp Gly Arg Gly Thr Leu Val Thr Val Ser
                245                 250                 255     
Ser
    


<210> 2
<211> 263
<212> PRT
<213> Artificial Sequence

<220> 
<223> PGT9-scFV

<400> 2
Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly Ser Pro Gly Gln
 1               5                  10                  15      
Ser Ile Thr Ile Ser Cys Asn Gly Thr Ser Asn Asp Val Gly Gly Tyr
            20                  25                  30          
Glu Ser Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Val
        35                  40                  45              
Val Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Ser Asn Arg Phe
    50                  55                  60                  
Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu
65                  70                  75                  80  
Gln Ala Glu Asp Glu Gly Asp Tyr Tyr Cys Lys Ser Leu Thr Ser Thr
                85                  90                  95      
Arg Arg Arg Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Arg Ser
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln
        115                 120                 125             
Arg Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Ser Ser Leu
    130                 135                 140                 
Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Gln Gly Met
145                 150                 155                 160 
His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Val Ala Phe
                165                 170                 175     
Ile Lys Tyr Asp Gly Ser Glu Lys Tyr His Ala Asp Ser Val Trp Gly
            180                 185                 190         
Arg Leu Ser Ile Ser Arg Asp Asn Ser Lys Asp Thr Leu Tyr Leu Gln
        195                 200                 205             
Met Asn Ser Leu Arg Val Glu Asp Thr Ala Thr Tyr Phe Cys Val Arg
    210                 215                 220                 
Glu Ala Gly Gly Pro Asp Tyr Arg Asn Gly Tyr Asn Tyr Tyr Asp Phe
225                 230                 235                 240 
Tyr Asp Gly Tyr Tyr Asn Tyr His Tyr Met Asp Val Trp Gly Lys Gly
                245                 250                 255     
Thr Thr Val Thr Val Ser Ser
            260             


<210> 3
<211> 269
<212> PRT
<213> Artificial Sequence

<220> 
<223> PGT145-scFV

<400> 3
Glu Val Val Ile Thr Gln Ser Pro Leu Phe Leu Pro Val Thr Pro Gly
 1               5                  10                  15      
Glu Ala Ala Ser Leu Ser Cys Lys Cys Ser His Ser Leu Gln His Ser
            20                  25                  30          
Thr Gly Ala Asn Tyr Leu Ala Trp Tyr Leu Gln Arg Pro Gly Gln Thr
        35                  40                  45              
Pro Arg Leu Leu Ile His Leu Ala Thr His Arg Ala Ser Gly Val Pro
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80  
Ser Arg Val Glu Ser Asp Asp Val Gly Thr Tyr Tyr Cys Met Gln Gly
                85                  90                  95      
Leu His Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
            100                 105                 110         
Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
        115                 120                 125             
Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly
    130                 135                 140                 
Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Asn Ser Phe Ser Asn
145                 150                 155                 160 
His Asp Val His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp
                165                 170                 175     
Met Gly Trp Met Ser His Glu Gly Asp Lys Thr Gly Leu Ala Gln Lys
            180                 185                 190         
Phe Gln Gly Arg Val Thr Ile Thr Arg Asp Ser Gly Ala Ser Thr Val
        195                 200                 205             
Tyr Met Glu Leu Arg Gly Leu Thr Ala Asp Asp Thr Ala Ile Tyr Tyr
    210                 215                 220                 
Cys Leu Thr Gly Ser Lys His Arg Leu Arg Asp Tyr Phe Leu Tyr Asn
225                 230                 235                 240 
Glu Tyr Gly Pro Asn Tyr Glu Glu Trp Gly Asp Tyr Leu Ala Thr Leu
                245                 250                 255     
Asp Val Trp Gly His Gly Thr Ala Val Thr Val Ser Ser
            260                 265                 


<210> 4
<211> 42
<212> PRT
<213> Artificial Sequence

<220> 
<223> 4-1BB signaling domain

<400> 4
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 5
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD3-zeta signaling domain

<400> 5
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 6
<211> 257
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 6
Gln Ser Ala Leu Thr Gln Pro Pro Ser Ala Ser Gly Ser Pro Gly Gln
 1               5                  10                  15      
Ser Ile Thr Ile Ser Cys Thr Gly Thr Ser Asn Asn Phe Val Ser Trp
            20                  25                  30          
Tyr Gln Gln His Ala Gly Lys Ala Pro Lys Leu Val Ile Tyr Asp Val
        35                  40                  45              
Asn Lys Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser
    50                  55                  60                  
Gly Asn Thr Ala Ser Leu Thr Val Ser Gly Leu Gln Thr Asp Asp Glu
65                  70                  75                  80  
Ala Val Tyr Tyr Cys Gly Ser Leu Val Gly Asn Trp Asp Val Ile Phe
                85                  90                  95      
Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Ser Gly Gly Gly Gly Ser
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Pro Gln Leu Gln Glu
        115                 120                 125             
Ser Gly Pro Thr Leu Val Glu Ala Ser Glu Thr Leu Ser Leu Thr Cys
    130                 135                 140                 
Ala Val Ser Gly Asp Ser Thr Ala Ala Cys Asn Ser Phe Trp Gly Trp
145                 150                 155                 160 
Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Val Gly Ser Leu Ser
                165                 170                 175     
His Cys Ala Ser Tyr Trp Asn Arg Gly Trp Thr Tyr His Asn Pro Ser
            180                 185                 190         
Leu Lys Ser Arg Leu Thr Leu Ala Leu Asp Thr Pro Lys Asn Leu Val
        195                 200                 205             
Phe Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala Thr Tyr Tyr
    210                 215                 220                 
Cys Ala Arg Phe Gly Gly Glu Val Leu Arg Tyr Thr Asp Trp Pro Lys
225                 230                 235                 240 
Pro Ala Trp Val Asp Leu Trp Gly Arg Gly Thr Leu Val Thr Val Ser
                245                 250                 255     
Ser
    


<210> 7
<211> 263
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 7
Gln Ser Ala Leu Thr Gln Pro Ala Ser Val Ser Gly Ser Pro Gly Gln
 1               5                  10                  15      
Ser Ile Thr Ile Ser Cys Asn Gly Thr Ser Asn Asp Val Gly Gly Tyr
            20                  25                  30          
Glu Ser Val Ser Trp Tyr Gln Gln His Pro Gly Lys Ala Pro Lys Val
        35                  40                  45              
Val Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val Ser Asn Arg Phe
    50                  55                  60                  
Ser Gly Ser Lys Ser Gly Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu
65                  70                  75                  80  
Gln Ala Glu Asp Glu Gly Asp Tyr Tyr Cys Lys Ser Leu Thr Ser Thr
                85                  90                  95      
Arg Arg Arg Val Phe Gly Thr Gly Thr Lys Leu Thr Val Leu Arg Ser
            100                 105                 110         
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln
        115                 120                 125             
Arg Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Ser Ser Leu
    130                 135                 140                 
Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Gln Gly Met
145                 150                 155                 160 
His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Val Ala Phe
                165                 170                 175     
Ile Lys Tyr Asp Gly Ser Glu Lys Tyr His Ala Asp Ser Val Trp Gly
            180                 185                 190         
Arg Leu Ser Ile Ser Arg Asp Asn Ser Lys Asp Thr Leu Tyr Leu Gln
        195                 200                 205             
Met Asn Ser Leu Arg Val Glu Asp Thr Ala Thr Tyr Phe Cys Val Arg
    210                 215                 220                 
Glu Ala Gly Gly Pro Asp Tyr Arg Asn Gly Tyr Asn Tyr Tyr Asp Phe
225                 230                 235                 240 
Tyr Asp Gly Tyr Tyr Asn Tyr His Tyr Met Asp Val Trp Gly Lys Gly
                245                 250                 255     
Thr Thr Val Thr Val Ser Ser
            260             


<210> 8
<211> 269
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 8
Glu Val Val Ile Thr Gln Ser Pro Leu Phe Leu Pro Val Thr Pro Gly
 1               5                  10                  15      
Glu Ala Ala Ser Leu Ser Cys Lys Cys Ser His Ser Leu Gln His Ser
            20                  25                  30          
Thr Gly Ala Asn Tyr Leu Ala Trp Tyr Leu Gln Arg Pro Gly Gln Thr
        35                  40                  45              
Pro Arg Leu Leu Ile His Leu Ala Thr His Arg Ala Ser Gly Val Pro
    50                  55                  60                  
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80  
Ser Arg Val Glu Ser Asp Asp Val Gly Thr Tyr Tyr Cys Met Gln Gly
                85                  90                  95      
Leu His Ser Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys
            100                 105                 110         
Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly
        115                 120                 125             
Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly
    130                 135                 140                 
Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Asn Ser Phe Ser Asn
145                 150                 155                 160 
His Asp Val His Trp Val Arg Gln Ala Thr Gly Gln Gly Leu Glu Trp
                165                 170                 175     
Met Gly Trp Met Ser His Glu Gly Asp Lys Thr Gly Leu Ala Gln Lys
            180                 185                 190         
Phe Gln Gly Arg Val Thr Ile Thr Arg Asp Ser Gly Ala Ser Thr Val
        195                 200                 205             
Tyr Met Glu Leu Arg Gly Leu Thr Ala Asp Asp Thr Ala Ile Tyr Tyr
    210                 215                 220                 
Cys Leu Thr Gly Ser Lys His Arg Leu Arg Asp Tyr Phe Leu Tyr Asn
225                 230                 235                 240 
Glu Tyr Gly Pro Asn Tyr Glu Glu Trp Gly Asp Tyr Leu Ala Thr Leu
                245                 250                 255     
Asp Val Trp Gly His Gly Thr Ala Val Thr Val Ser Ser
            260                 265                 


<210> 9
<211> 42
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 9
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 10
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 10
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 11
<211> 23
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 11
Met Cys Pro Gln Lys Leu Thr Ile Ser Trp Phe Ala Ile Val Leu Leu
 1               5                  10                  15      
Val Ser Pro Leu Met Ala Met
            20              


<210> 12
<211> 69
<212> PRT
<213> Artificial Sequence

<220> 
<223> Homo sapiens

<400> 12
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala
 1               5                  10                  15      
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly
            20                  25                  30          
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile
        35                  40                  45              
Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val
    50                  55                  60                  
Ile Thr Leu Tyr Cys
65                  


<210>  13
<211>  346
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220> 
<221>  misc_feature
<223>  MND promoter

<220> 
<221>  misc_feature
<223>  modified moloney murine leukemic virus promoter with
       myleloproliferative sarcoma virus enhancer

<400>  13
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagat                     346


<210>  14
<211>  938
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Long human EF1a promoter

<400>  14
gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg       60

ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa      120

gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg      180

aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc      240

tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt      300

ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt      360

ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg      420

gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc      480

tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt      540

cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa      600

aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg      660

cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc      720

acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt      780

atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact      840

tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc      900

ctcagacagt ggttcaaagt ttttttcttc catttcag                              938


<210>  15
<211>  231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  short human EF1a promoter

<400>  15
aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg       60

gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag      120

tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc      180

agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac a               231
